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CMV QuAnt RT-qPCR Kit
LaborantTM CMV QuAnt RT-qPCR Kit is used for quantification of the Cytomegalovirus with PCR amplification in a single tube using gene-specific primers. The kit allows to achieve RT-qPCR result in around 40 minutes and may vary according to the Ramp Rate of the device. Rapid diagnosis with the kit is achieved via real-time PCR (qPCR) (RT-qPCR) targeting CMV specific gene fragments. The oligonucleotide set targeting human RPP-30 gene functions as a control of the sampling, nucleic acid extraction and inhibition. All standards (CMV-S E3, CMV-S E4, CMV-S E5, CMV-S E6) have been meticulously designed and formulated to mimic CMV positive human samples by making quantitative dilutions of CMV gene fragments. They were previously tested and found to be negative for HCV RNA, HIV-1 RNA, antibodies to HIV-1 and HIV-2, HBsAg, antibodies to HCV, and antibodies to HTLV I-II. They have been calibrated against the World Health Organization (WHO) International Standard for CMV DNA and the assigned values are reported as International Units per milliliter (IU/mL).