Quantitative detection and viral load monitoring of human Adenovirus DNA (subtypes A, B1, B2, C, D, E, F, G) by real-time PCR.
Quantitative detection of Adenovirus DNA in nucleic acid extracts from human clinical samples including nasopharyngeal and oropharyngeal swabs, bronchoalveolar lavage, stool and urine. Viral load monitoring in allogeneic haematopoietic stem cell transplant recipients and in surveillance following solid organ transplantation.
Quantitative reporting is performed in copies/mL. Trend analysis (serial samples) is clinically more meaningful than a single absolute value.
| Target gene | Hexon (conserved region, covers 8 subtypes) |
| Internal control | β-Globin (co-amplification on HEX channel) |
| Format | 1-mix mastermix · 4-channel readout (FAM, HEX) |
| Reaction volume | 10 µL total |
| Cycle time | ~50 min |
| Standard curve | 4-point, 10³ – 10⁶ copies/µL |
| Oligo Mix | 1 × 24 µL · primer + probe |
| Uniqplex Enzyme Mix | 1 × 120 µL |
| NTC | 1 × 1000 µL · negative control |
| Standards (4 points) | 4 × 50 µL · 10³ – 10⁶ copies/µL |
48- and 96-reaction packs are also available — see IFU §3.
The typical LoD is in the range of 50–500 copies/mL in extracted samples. The linear amplification range extends approximately 5–6 log₁₀ above the LoD. Kit-specific performance values and cross-reactivity data are documented in the performance evaluation report.
| Before use | −20 °C · until expiry date |
| After opening | −20 °C · 4 months (including freeze-thaw) |
| Freeze-thaw cycle | Maximum 3 |
Class C IVD device. CE marked under the transitional provisions of Regulation (EU) 2017/746. The conformity assessment process under (EU) 2017/746 (IVDR) is ongoing. TİTCK registered. For in vitro diagnostic use only — for laboratory and professional use.